Quantitative in vivo receptor binding III: Tracer kinetic modeling of muscarinic cholinergic receptor binding.
نویسندگان
چکیده
منابع مشابه
Quantitative in vivo receptor binding III: Tracer kinetic modeling of muscarinic cholinergic receptor binding.
A tracer kinetic method is developed for the in vivo estimation of high-affinity radioligand binding to central nervous system receptors. Ligand is considered to exist in three brain pools corresponding to free, nonspecifically bound, and specifically bound tracer. These environments, in addition to that of intravascular tracer, are interrelated by a compartmental model of in vivo ligand distri...
متن کاملQuantitative in vivo receptor binding. I. Theory and application to the muscarinic cholinergic receptor.
A novel approach to in vivo receptor binding experiments is presented which allows direct quantitation of binding site densities. The method is based on an equilibrium model of tracer uptake and is designed to produce a static distribution proportional to receptor density and to minimize possible confounding influences of regional blood flow, blood-brain barrier permeability, and nonspecific bi...
متن کاملQuantitative in vivo receptor binding. II. Autoradiographic imaging of muscarinic cholinergic receptors.
The in vivo distribution of [3H]scopolamine in rat brain following establishment of constant, saturating arterial tracer concentrations was examined with the use of quantitative autoradiography. The equilibrium drug distribution, studied 240 min after initiation of tracer infusion, was highly correlated with the regional density of muscarinic receptor sites determined in vitro in the same anima...
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Binding sites with high affinity and specificity for [(3)H]quinuclidinyl benzilate (QNB) are present in homogenates of rat brain. The characteristics of the binding sites resemble those of muscarinic cholinergic receptors. Specific binding is saturable with respect to [(3)H]QNB and tissue concentration and is time-, temperature-, and pH-dependent. The bimolecular rate of association (2.0 x 10(8...
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Introduction : The innate immune system as the first line of defense against the pathogens recognizes pathogen-associated molecular patterns (PAMPs) by Toll-Like Receptors (TLRs). Interaction of bacterial PAMPs by TLRs results in activation of innate and acquired immunity. FimH adhesin, a minor component of type 1 fimbriae encoded by Uropathogenic Escherichia coli (UPEC) is a PAMP of TLR4 tha...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1985
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.82.19.6711